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Biological Treatment

Advanced biological wastewater treatment systems including MBBR, SBR, MBR, EGSB, and traditional activated sludge reactors for effective organic matter removal and nutrient reduction.

Scientific Modelling & Simulation

Behind the design sits a full modelling toolkit — CFD, process simulation, biokinetic (ASM/ADM), reaction-kinetics, hydraulic, limnological and data-driven digital-twin modelling. We pick, or combine, the disciplines that answer your question and validate them against real data.

Explore Scientific Modelling

How Biological Treatment Works

Microbial Degradation Process

Biological treatment harnesses the power of microorganisms to break down organic pollutants in wastewater. Different technologies optimise this process for specific applications, flow rates, and treatment requirements.

1

Pre-treatment

Screening & grit removal

2

Biological

Microbial degradation

3

Clarification

Solids separation

4

Tertiary

Polishing treatment

5

Discharge

Clean water out

Biological Treatment — Microbial Degradation
Microorganisms break down organic pollutants through aerobic or anaerobic metabolic pathways. Bacteria, protozoa, and other microbes convert BOD and COD into biomass, carbon dioxide, water, and — under anaerobic conditions — methane-rich biogas. This is the core treatment stage where 80–99% of organic load is removed.
BOD Removal 90–99%Aerobic / AnaerobicMBBR / SBR / MBR / EGSBNutrient RemovalBiogas Recovery

Moving Bed Biofilm Reactor

MBBR

Uses biomass carriers for microorganisms to degrade contaminants. Carriers move freely in reactor ensuring effective contact. High efficiency and low operating requirements for industrial and municipal WWTPs.

Free-Floating Carriers

High surface area

Continuous Movement

Optimal contact

Biofilm Growth

Protected biomass

Space-Efficient Design

Smaller footprint than conventional activated sludge systems

No Sludge Recycle

Simpler operation without return activated sludge pumping

Shock Load Resistant

Protected biofilm handles variable loading conditions

Easy Upgrade

Can be retrofitted into existing tanks

Sequencing Batch Reactor

SBR

Sequential advanced biological treatment where all process stages (aeration, settling, decanting) occur in single tank. Automatic control enables nitrification, denitrification, and dephosphatation. Highly resistant to variable flows and loads, ideal for industrial facilities.

SBR Cycle Phases

Fill

Wastewater inlet

60 – 120 min

React

Aeration + mixing

120 – 240 min

Settle

Solids separation

45 – 90 min

Decant

Clean water out

30 – 60 min

Idle

Standby / waste sludge

15 – 30 min

3-in-1
Process in Single Tank
N/DN/DP
Nitrification/Denitrification/Dephosphatation
High
Flow Variation Resistance

Membrane Bioreactor

MBR

Combines biological decomposition with membrane filtration. High efficiency enables innovative water recovery for reuse. Compact design with high-quality effluent.

Superior Effluent Quality

Produces water suitable for reuse with very low TSS and turbidity

Smallest Footprint

Eliminates secondary clarifier; most compact advanced biological treatment

High Biomass Concentration

MLSS up to 12 g/L enables smaller reactor volumes

Water Reuse Ready

Effluent meets stringent standards for direct reuse applications

Expanded Granular Sludge Bed

EGSB

High-rate anaerobic treatment using granular sludge in an upflow configuration. EGSB handles very high organic loads (10–30 kg COD/m³·d), produces biogas rich in methane (CH₄ 65–75%, CO₂ 25–35%), and generates minimal excess sludge. Ideal for breweries, distilleries, food processing, and pharmaceutical wastewaters with high COD.

Biogas Recovery

0.3–0.5 m³ biogas per kg CODremoved. Calorific value ~23 MJ/m³. Usable for boiler fuel, CHP, or process heat.

Granular Sludge

Dense granules (1–4 mm) with high methanogenic activity. Upflow velocity 3–10 m/h. Sludge volume index <30 mL/g.

Low Lifecycle Cost

No aeration energy. Low chemical demand. Sludge production 5–10% of aerobic systems. Positive energy balance possible.

Temperature Flexibility

Mesophilic (30–40°C) and thermophilic (50–60°C) operation. Insulated reactors with heat recovery from biogas combustion.

Conventional Activated Sludge Process

ASP

The traditional workhorse of biological wastewater treatment. Suspended-growth aerobic process with primary clarifier, aeration tank, and secondary clarifier. Reliable, well-understood, and efficient for large continuous flows with minimal variation.

Field-Proven Technology

Over 100 years of operational history. Extensive design guidelines and operator familiarity worldwide.

Lowest Capital

Simple tanks and diffusers. No membranes, carriers, or complex control systems. Lowest initial investment.

Large Flows

Ideal for >1,000 m³/d municipal plants. Proven at scales exceeding 1 MLD with minimal operator intervention.

Easy O&M

Standard aeration equipment. Sludge volume index (SVI) monitoring. Conventional RAS/WAS pumping.

Kinetic Foundations

The Equations Behind Every Aeration Tank

Monod Growth Kinetics

Specific growth rate of heterotrophs scales with the limiting substrate concentration: μ = μmax·S / (KS + S). For municipal wastewater at 20 °C: μmax ≈ 6 d−1, half-saturation constant KS = 20–80 mg COD/L, yield Y = 0.4–0.6 g VSS/g COD, endogenous decay b = 0.05–0.10 d−1. Minimum SRT for stable operation: SRTmin = 1 / (Y·μmax·S/(KS+S) − b) — this is why nitrifying plants need SRT ≥ 8–15 d (nitrifier μmax is only ~0.7 d−1).

Oxygen Mass Transfer

Field oxygen transfer rate: OTR = α·F·(SOTR)·[(β·CS20* − CL) / CS20*]·1.024(T−20). Fine-bubble diffusers achieve SOTE = 6–8%/m of submergence in clean water; the α-factor for activated sludge falls to 0.4–0.7. Oxygen demand: 1.0–1.5 kg O2/kg BODremoved for carbonaceous removal, plus 4.57 kg O2/kg N for nitrification. Energy cost is dominated by this term — aeration is 50–70% of plant electricity.

Reactor Mass Balance & SRT

For a CSTR with biomass recycle: SRT = V·X / (Qw·Xw + Qe·Xe). Effluent substrate at steady state: S = KS·(1 + b·SRT) / [SRT·(Y·k − b) − 1]. SRT is the single most important design variable — it sets effluent quality, biomass yield, sludge production, and which microbial guilds (heterotrophs, nitrifiers, anammox) survive in the reactor.

Temperature Correction

Most biological rates follow the modified Arrhenius form kT = k20·θ(T−20) with θ = 1.04 for BOD removal, 1.08–1.12 for nitrification, 1.10–1.15 for denitrification, and 1.06–1.10 for methanogenesis. Below 10 °C nitrification effectively halts; below 15 °C anaerobic granular reactors lose 30–50% of design capacity unless heated.

Design Parameters & Operating Ranges

Typical specifications for sizing advanced biological treatment equipment

Hydraulic Retention Time

MBBR: 4 – 12 h
SBR: 8 – 24 h (cycle time)
MBR: 6 – 18 h
EGSB: 4 – 24 h
Conventional: 12 – 36 h

Solids Retention Time (SRT)

MBBR: N/A (biofilm)
SBR: 10 – 30 d
MBR: 15 – 40 d
EGSB: 30 – 100 d
Conventional: 5 – 15 d

F/M Ratio & Loading

F/M (aerobic): 0.05 – 0.3 kg BOD/kg MLSS·d
OLR (aerobic): 0.5 – 5 kg COD/m³·d
OLR (EGSB): 10 – 30 kg COD/m³·d
N-loading: 0.05 – 0.3 kg NH₄-N/kg MLSS·d

MLSS / MLVSS

MBBR: 3 – 5 g/L (suspended)
SBR: 3 – 5 g/L
MBR: 8 – 12 g/L
EGSB: 15 – 40 g/L (granular)
Conv.: 2 – 4 g/L

Temperature & pH

Mesophilic: 15 – 35°C (opt. 25°C)
Thermophilic: 45 – 60°C (EGSB)
pH (aerobic): 6.5 – 8.5
pH (anaerobic): 6.5 – 7.5

Oxygen & Energy

O₂ requirement: 1.0 – 1.5 kg O₂/kg BOD
SOTE: 20 – 35% (fine bubble)
Energy (aerobic): 0.5 – 2 kWh/kg COD
Biogas yield: 0.3 – 0.5 m³/kg CODremoved
Engineering note: All parameters are typical ranges for municipal and industrial wastewater at 20°C. Kinetics slow by 20–30% below 10°C; compensate with longer HRT or higher MLSS. Pilot testing → is recommended for high-strength or toxic wastewaters.

Automation & Control

SCADA Integration Available

SCADA & Control Systems

Our advanced biological treatment systems feature advanced automation capabilities with SCADA integration. Monitor DO levels, control aeration, manage SBR cycles, and track biomass health from a centralised control interface. Double-redundant architecture ensures continuous operation for critical applications.

DO MonitoringAeration ControlSBR Cycle ManagementAlarm & Reporting
Discover More About SCADA

Technology Comparison

Select the Right Biological Treatment for Your Wastewater

FeatureMBBRSBRMBREGSBConventional ASP
FootprintSmallMediumSmallestSmallLarge
Energy UseLowMediumMedium-HighVery LowMedium
HRT Range4 – 12 h8 – 24 h6 – 18 h4 – 24 h12 – 36 h
MLSS Range3 – 5 g/L3 – 5 g/L8 – 12 g/L15 – 40 g/L2 – 4 g/L
Organic Loading1 – 5 kg COD/m³·d0.5 – 2 kg COD/m³·d2 – 8 kg COD/m³·d10 – 30 kg COD/m³·d0.3 – 1 kg COD/m³·d
Nutrient Removal
N / P
~ Partial✓✓✓ Full✓✓ Good✗ None~ Partial
Water Reuse Quality✗ No~ Polishing needed✓✓✓ Direct reuse✗ No✗ No
Biogas Production✗ No✗ No✗ No✓✓✓ High yield✗ No
Shock Load Resistance✓✓✓ Excellent✓✓ Good✓ Moderate✓✓ Good✗ Poor
Temp. Range10 – 35°C10 – 35°C10 – 35°C25 – 40°C10 – 35°C
pH Range6.5 – 8.56.5 – 8.56.5 – 8.56.5 – 7.56.5 – 8.5

Which Technology for Your Industry?

Match your wastewater characteristics and operational constraints to the optimal advanced biological treatment

Industry / ApplicationWastewater ProfileRecommended TechnologyKey Rationale
Food & Beverage
High COD, variable load
COD 2,000–15,000 mg/L, FOG, seasonalMBBR or EGSBMBBR resists shock loads; EGSB recovers biogas from high COD
Brewery / Distillery
Very high COD, warm
COD 5,000–50,000 mg/L, warm (>25°C)EGSBAnaerobic pre-treatment removes 70–90% COD + biogas output. See brewery case study →
Pharmaceutical
Strict effluent, reuse
Variable, trace APIs, tight N/P limitsMBRSuperior effluent for reuse or tight discharge. Complete solids retention.
Municipal WWTP
Nutrient removal required
COD 300–600 mg/L, TN/TP limitsSBR or ConventionalSBR enables N/DN/DP in single tank. Conventional for >5 MLD.
Chemical / Industrial
Toxic shocks, intermittent
Variable pH, inhibitors, batch dischargesMBBRProtected biofilm resists toxic shocks. No sludge washout risk.
Meat / Poultry
High protein, blood, fat
COD 3,000–10,000 mg/L, high TKNMBBR + SBRMBBR for COD; SBR for nitrification/denitrification of high TKN.
Dairy
High fat, lactose
COD 1,000–5,000 mg/L, FOGMBBRCompact footprint. Handles FOG with DAF pre-treatment.

Compliance & Benefits

Meeting Discharge Standards

Regulatory Compliance

Systems designed to meet BOD, COD, TSS, nitrogen, and phosphorus discharge limits per local regulations. Pilot testing validates effluent quality before full-scale commitment.

Environmental Impact

Reduce organic pollution load to receiving waters; recover energy through anaerobic processes

Operating Efficiency Gains

Low energy consumption, minimal chemical requirements, and reduced sludge disposal requirements through efficient dewatering.

Scalable Solutions

Modular designs and containerised configurations allow easy capacity expansion and rapid deployment.

Related Equipment

Complete Your Treatment System

DAF Systems

Pre-treatment flotation for solids and oil removal before biological stages.

Explore Physico-Chemical

Lamella Clarifier

Clarification for biological sludge separation and effluent polishing.

View Lamella Clarifier

Water Aeration

Aeration systems for biological processes and oxygen transfer.

Water Aeration

Sludge Dewatering

Multi-disc presses, belt presses, and centrifuges for biological sludge volume reduction.

View Sludge Management

Storage Tanks

Equalization and buffer tanks for flow balancing and emergency storage in biological systems.

Storage Tanks

Process Tanks

Aeration tanks, contact tanks, and equalization vessels for advanced biological treatment processes.

Process Tanks

Loading Rates, Sizing & Operational Envelopes

Engineering parameters for biological process design and equipment selection.

ParameterMBBRSBRMBREGSBCAS
Design HRT (h)4–128–246–150.5–34–8
OLR (kg BOD/m3·d)2–80.5–2.52–610–250.3–1.0
MLSS (g/L)3–5 (film)2.5–4.08–1215–40 (granules)2–4
F/M ratio (kg BOD/kg MLSS·d)0.5–2.00.05–0.150.15–0.40.2–0.5
SRT (d)10–3010–3015–4030–1003–15
SOUR (mg O2/g MLVSS·h)15–4010–3010–2515–40
Specific energy (kWh/m3)0.15–0.350.20–0.450.50–1.200.05–0.150.25–0.50
Sludge yield (kg DS/kg BODrem)0.50–0.750.60–0.900.70–1.000.05–0.150.60–0.90
Typical effluent BOD (mg/L)10–2510–20<550–20015–30

These ranges are indicative at 20°C. Temperature correction (θ = 1.03–1.08) applies for cold-climate design. Reynolds & Bauhm calibrates design parameters against pilot data or plant benchmarking for each project.

Energy Consumption & Carbon Footprint

Aeration Energy Optimisation

Aeration accounts for 50–70% of total biological treatment energy. Fine-bubble diffusers achieve 4–6 kg O2/kWh vs 1.5–2.5 for coarse bubble. DO control with VFD blowers and automated valves typically reduces aeration energy 15–30%.

Membrane Air Scouring

MBR membrane air scouring consumes 0.15–0.30 kWh/m3. Cyclic aeration, relaxation modes, and energy-efficient flat-sheet configurations reduce this by 20–40% compared to legacy hollow-fibre systems.

Anaerobic Energy Recovery

EGSB and UASB biogas yield 0.25–0.35 Nm3 CH4/kg CODrem. CHP conversion produces 1.5–2.5 kWh electricity per m3 biogas, often making anaerobic plants net energy positive.

Carbon Footprint Comparison

Carbon intensity (kg CO2e/m3 treated): CAS 0.35–0.60; MBBR 0.25–0.45; MBR 0.50–0.90; EGSB −0.10 to +0.15 (net negative when biogas recovered). Scope 3 sludge transport adds 0.05–0.15 kg CO2e/m3.

Sludge Production, Yield & Management

Observed Yield Coefficients

The observed sludge yield Yobs decreases with increasing SRT due to endogenous respiration. At 20°C:

  • SRT = 3 d: Yobs ≈ 0.80 kg TSS/kg BODrem
  • SRT = 10 d: Yobs ≈ 0.65 kg TSS/kg BODrem
  • SRT = 20 d: Yobs ≈ 0.50 kg TSS/kg BODrem
  • SRT = 30 d: Yobs ≈ 0.40 kg TSS/kg BODrem

Px = Yobs × Q × (S0 − S)

Where Px = waste sludge production (kg TSS/d); Q = flow (m3/d); S0−S = BOD removed (kg/m3).

Sludge Management Pathways

  • Thickening: Gravity (2–4% DS) or dissolved-air flotation (3–5% DS)
  • Dewatering: Belt press (15–22% DS); centrifuge (18–25% DS); multi-disc screw (12–20% DS)
  • Thermal drying: Paddle dryer or solar drying to 70–90% DS for landfill or incineration
  • Anaerobic digestion: 30–50% VS destruction; biogas recovery; digestate to land
  • Composting: Mixed with green waste; 6–8 week windrow; pathogen reduction

Reynolds & Bauhm designs sludge management as an integrated part of the biological treatment train, not an afterthought.

Not Sure Which Biological Technology Fits?

Our process engineers can run treatability studies, jar testing, and bench-scale trials to validate technology selection for your specific wastewater. Download our Biological Treatment Selection Guide or request a pilot study to generate design data with confidence.

Industries We Serve

Our expertise spans multiple industries with sector-specific water treatment solutions.